Monoclonal Anti-Dihydropyridine Receptor (alpha2 Subunit) antibody produced in mouse, clone 20A, ascites fluid, buffered aqueous solution

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Western Blotting (1 paper)

Use of this antibody for immunoblot analysis will allow for detection of a 220 kDa band (native conditions) or a 143 kDa band (denaturing conditions).Use of this antibody for immunohistochemistry of muscle tissue will demonstrate double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines are observed.

Monoclonal Anti-Dihydropyridine Receptor (α2 Subunit) antibody produced in mouse has been used in:immunofluorescent histochemistrysurface plasmon resonance binding immunohistochemistry (IHC) immunoblotting

Biochem/physiol Actions

Dihydropyridine Receptor (DHFR) α1subunit is the voltage-sensor. DHPR interacts with ryanodine receptor (RyR) and also mediates excitation-contraction (EC) coupling in cardiac and skeletal muscles. Mutation in the DHPR (E1014K) affects its affinity and selectivity towards calcium permeation. Additionally, it also plays a key role in the synaptic transmission.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Dihydropyridine Receptor (DHPR) is a heteropentamer calcium ion channel and comprises of α1,α2/δ,βandγsubunits. It is localized in the skeletal muscle in the t-tubule membrane. The α2subunit of DHPR is a glycosylated subunit and is anchored on the membrane.

Immunogen

Rabbit dihydropyridine (DHP) receptors, (α2 subunit)

Other Notes

This mouse monoclonal IgG2a antibody is supplied as a mouse ascites diluted in phosphate buffered saline containing 0.05% sodium azide as a preservative.

Physical form

Solution in phosphate buffered saline containing 0.05% sodium azide.

Specificity

Reacts specifically with the α₂ subunit of the DHP receptor. Strong reactivity is observed with the α₂ subunit in rat, mouse, guinea pig, rabbit skeletal and cardiac muscle, and rat brain and lung tissue. Weaker reactivity is observed with rat kidney, liver and spleen tissues. By immunohistochemistry, double rows of discrete punctate staining represent pairs of triads on the opposing sides of the Z-lines.